Ku protein - Ku protein - qaz.wiki

Although the intensity of the Ku70 band in the input mixture was greater than that for Ku80, roughly equal amounts of both were co-precipitated by the anti-HA and anti-p23 antibodies. The 12/23 rule is a critical step for regulation of V(D)J recombination. To date, only the RAG proteins and high mobility group protein 1 or 2 have been implicated in 12/23 regulation. Through protein fractionation and biochemical experiments, we find that Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) modulate RAG-mediated cleavage. Modulation of cleavage by Ku70/80 The Ku70/80 heterodimer is the regulatory subunit of the DNA-dependent protein kinase (DNA-PK) and its DNA-binding activity mediates DNA double-strand breaks repair. Although Ku80 was recently 2016-7-1 · The Ku70/80 heterodimer protein plays a pivotal role in the NHEJ process.

Ku70 80

/ Fransson, Johan; Borrebaeck, Carl.. I: International Journal of Cancer, Vol. 119, Nr. 10, 2006, s. 2492-2496. Ku70/80 with DSBs, as well as the post-repair dissociation of Ku70/80 from the DNA, and evaluate the pathways and factors that potentially influence this removal. 3. 14375 Ensembl ENSG00000196419 ENSMUSG00000022471 UniProt P12956 P23475 RefSeq (mRNA) NM_001469 NM_001288976 NM_001288977 NM_001288978 NM_010247 RefSeq (protein) NP_001275905 NP_001275906 NP_001275907 NP_001460 NP_001275905.1 NP_001460.1 NP_034377 Location (UCSC) Chr 22: 41.62 – 41.66 Mb Chr 15: 81.99 – 82.04 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Ku70 is a protein that Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. 2012-02-10 · Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex.

Modifying DNA ends.

Generated from HitPredict database 03Aug2020

Ku70 or Ku80 overexpression Ku70 — белок, в человеческом организме кодируемый геном XRCC6. Содержание. 1 Функция; 2 Старение; 3 Номенклатура; 4 Взаимодействия; 5 Примечания. Функция[править | править код].

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Although Ku80 was recently The Ku70/80 heterodimer (Ku), the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs), DNA ligase IV (LigIV), XRCC4 and XLF form a long-range synaptic complex, in which the DNA ends The Ku70/80 crystal structure (Fig. 1B) shows that the two subunits dimerize through the central domain to form a ring capable of accommodating two turns of double-stranded DNA (approximately 14 base pairs) . This ring, consisting of intertwined strands of both Ku70 and Ku80, is lined with positively charged residues positioned to interact with Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function.

A Ku70/Ku80 complex binds initially to two DNA ends at the DSB site, and then recruits a DNA‐dependent protein kinase (DNA‐PKcs), which has not been identified in plants.
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2492-2496. Ku70/80 with DSBs, as well as the post-repair dissociation of Ku70/80 from the DNA, and evaluate the pathways and factors that potentially influence this removal. 3. 14375 Ensembl ENSG00000196419 ENSMUSG00000022471 UniProt P12956 P23475 RefSeq (mRNA) NM_001469 NM_001288976 NM_001288977 NM_001288978 NM_010247 RefSeq (protein) NP_001275905 NP_001275906 NP_001275907 NP_001460 NP_001275905.1 NP_001460.1 NP_034377 Location (UCSC) Chr 22: 41.62 – 41.66 Mb Chr 15: 81.99 – 82.04 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Ku70 is a protein that Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

This complex has a Provided herein are methods for identifying and treating subjects having conditions involving aberrant Ku70/80 activity. In particular, the invention relates to Inhibition of the Ku DNA end-binding activity by transfection with the C-terminal Ku80 expression gene suppressed cell proliferation. Ku70 or Ku80 overexpression Ku70 — белок, в человеческом организме кодируемый геном XRCC6. Содержание.
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Literature references 2005- Immuno technology

With high purity, high biological activity, high stability, and other superior features, you can use this Human Ku70 & Ku80 Heterodimer protein for relevant bioassay and related research. The relative intensity of the bands in Fig. 5 suggests that the interaction is specific for Ku70/80 heterodimers.

Genome-wide comprehensive analysis of human helicases

Den Ku70/Ku80 heterodimer binder till DSB slutar, rekrytera DNA-PKcs och p53 Bindande Odla celler till 80% konfluency (cirka 24 timmar). Ku70 visas i lila, Ku80 i blått och DNA-strängen i grönt. Identifierare Ku70 / Ku80 N-terminal alfa / beta-domän. FBF 1jeq EBI.jpg. av P Wallin — En cell befinner sig dock 80 procent av sin tid i S och G1 faserna under och Ku80. De två subenheterna fick namnen Ku70 respektive Ku80 eftersom det proteinet Ku70/80 fäster i vardera DNA- änden och drar ihop dem. DNA-prote- inkinas (DNA-PK) binder in och fosfo- rylerar sig själv (på Ser2056) och andra.

It was reported that activation of ATM- and Rad3-related (ATR) kinase by DSBs also occurs in an ATM-dependent manner. On the other hand, Ku70/80 is The dynamics of Ku70/80 and DNA-PKcs at DSBs induced by ionizing radiation is dependent on the complexity of damage. Reynolds P(1), Anderson JA, Harper JV, Hill MA, Botchway SW, Parker AW, O'Neill P. Author information: (1)Department of Oncology, Gray Institute for Radiation Oncology & Biology, University of Oxford, Oxford OX3 7DQ, UK. The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. Here, we describe intramolecular interactions of the Ku70 C-terminal domain, known as the SAP domain.